Argentinian Colace has been sidelined since early February due to a strained back muscle and has missed the last seven matches.
But manager Mark Robins must hand late fitness tests to three players, with defenders Paul McShane and Jason Shackell both nursing hamstring strains sustained in last week's draw at Cardiff and midfielder Jacob Mellis, on loan from Chelsea, nursing a similar problem.
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Barnsley vs Reading Live Streaming
Championship
15:00 GMT, March 19, 2011
Oakwell Stadium, England
